Book section : Chapter
Cysteine reactivity profiling to unveil redox regulation in phytopathogens
- Abstract:
- Reactivity-based chemical proteomics is a powerful technology based on the use of tagged chemicals that covalently react with surface-exposed residues on proteins in native proteomes. Reactivity profiling involves the purification, identification, and quantification of labeled peptides by LC-MS/MS. Here, we have detailed a protocol for reactivity profiling of Cys residues using iodoacetamide probes, displaying >1000 reactive Cys residues in the proteome of phytopathogen Pseudomonas syringae pv. tomato DC3000 (PtoDC3000). Comparative reactivity profiling of PtoDC3000 treated with or without hydrogen peroxide (H2O2) identified ~200 H2O2-sensitive Cys residues in antioxidant enzymes, metabolic enzymes, and transcription regulators. Interestingly, half of these H2O2-sensitive Cys residues are more reactive in response to H2O2 and several proteins have multiple Cys residues with opposite reactivities in response to H2O2 exposure.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Access Document
- Files:
-
-
(Preview, Accepted manuscript, pdf, 314.4KB, Terms of use)
-
- Publisher copy:
- 10.1007/978-1-0716-2079-3_9
Authors
+ Engineering and Physical Sciences Research Council
More from this funder
- Funder identifier:
- https://ror.org/0439y7842
- Grant:
- EP/X527245/1
- Publisher:
- Springer Nature
- Host title:
- Plant Proteases and Plant Cell Death: Methods and Protocols
- Pages:
- 105-117
- Series:
- Methods in Molecular Biology
- Series number:
- 2447
- Place of publication:
- New York, NY, USA
- Publication date:
- 2022-05-19
- DOI:
- EISSN:
-
1940-6029
- ISSN:
-
1064-3745
- Pmid:
-
35583776
- EISBN:
- 9781071620793
- ISBN:
- 9781071620786
- Language:
-
English
- Keywords:
- Subtype:
-
Chapter
- Pubs id:
-
1261813
- Local pid:
-
pubs:1261813
- Deposit date:
-
2025-04-29
Terms of use
- Copyright holder:
- Morimoto et al
- Copyright date:
- 2022
- Rights statement:
- © 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
- Notes:
- This is the accepted manuscript version of the paper. The final version is available online from Springer Nature at https://dx.doi.org/10.1007/978-1-0716-2079-3_9
If you are the owner of this record, you can report an update to it here: Report update to this record