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Assessment of an antibody-in-lymphocyte supernatant assay for the etiological diagnosis of pneumococcal pneumonia in children

Abstract:

New diagnostic tests for the etiology of childhood pneumonia are needed. We evaluated the antibody-in-lymphocyte supernatant (ALS) assay to detect immunoglobulin (Ig) G secretion from ex vivo peripheral blood mononuclear cell (PBMC) culture, as a potential diagnostic test for pneumococcal pneumonia. We enrolled 348 children with pneumonia admitted to Patan Hospital, Kathmandu, Nepal between December 2015 and September 2016. PBMCs sampled from participants were incubated for 48 h before harves...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.3389/fcimb.2019.00459

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Publisher:
Frontiers Media Publisher's website
Journal:
Frontiers in Cellular and Infection Microbiology Journal website
Volume:
9
Article number:
459
Publication date:
2020-01-17
Acceptance date:
2019-12-16
DOI:
EISSN:
2235-2988
Language:
English
Pubs id:
1086347
Local pid:
pubs:1086347
Deposit date:
2020-02-11

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