Strain-specific quantification of Wolbachia density in Aedes albopictus and effects of larval rearing conditions.

Journal article

The density of the endosymbiont Wolbachia can influence the expression of the crossing sterilities known as cytoplasmic incompatibility (CI), and also its rate of maternal transmission. Aedes albopictus mosquitoes contain a superinfection with the Wolbachia strains wAlbA and wAlbB. A strain-specific real-time quantitative PCR assay was developed and used to quantify relative Wolbachia strain densities within individual mosquitoes. The wAlbB strain was consistently found to be at higher densit...

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Authors: Dutton, T; Sinkins, S

• Publication status: Published
• Journal: Insect molecular biology
• Volume: 13
• Issue: 3
• Pages: 317-322
• Publication date: 2004-06-01
• DOI: 10.1111/j.0962-1075.2004.00490.x
• EISSN: 1365-2583
• ISSN: 0962-1075
• Language: English
• Keywords: Larva; Aedes; Electrophoresis, Agar Gel; Fluorometry; Bacterial Outer Membrane Proteins; Animals; Reproduction; Body Weights and Measures; Population Dynamics; Symbiosis; DNA Primers; Wolbachia; Population Density; Polymerase Chain Reaction