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Journal article

Continuous base identification for single-molecule nanopore DNA sequencing

Abstract:

A single-molecule method for sequencing DNA that does not require fluorescent labelling could reduce costs and increase sequencing speeds. An exonuclease enzyme might be used to cleave individual nucleotide molecules from the DNA, and when coupled to an appropriate detection system, these nucleotides could be identified in the correct order. Here, we show that a protein nanopore with covalently attached adapter molecule can continuously identify unlabelled nucleoside 5'-monophosphate molecule...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/NNANO.2009.12

Authors


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Institution:
Oxford Nanopore Technologies Ltd, Begbroke Science Park, Oxford, UK
Role:
Author
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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Chemistry Research Laboratory
Role:
Author
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Institution:
"Oxford Nanopore Technologies Ltd, Begbroke Science Park, Oxford, UK", "University of Oxford"
Department:
Mathematical,Physical & Life Sciences Division - Chemistry - Chemistry Research Laboratory
Role:
Author
More by this author
Institution:
Oxford Nanopore Technologies Ltd, Begbroke Science Park, Oxford, UK
Role:
Author
More by this author
Institution:
Oxford Nanopore Technologies Ltd, Begbroke Science Park, Oxford, UK
Role:
Author
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Funding agency for:
Bayley, H
European Commission More from this funder
Oxford Nanopore Technologies More from this funder
Publisher:
Macmillan Publishers Ltd. Publisher's website
Journal:
Nature Nanotechnology Journal website
Volume:
4
Issue:
4
Pages:
265-270
Publication date:
2009-04-01
DOI:
EISSN:
1748-3395
ISSN:
1748-3387
Language:
English
Subjects:
UUID:
uuid:124b71ab-f0ee-412a-afd6-436a58e63636
Local pid:
ora:4643
Deposit date:
2010-12-16

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