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Journal article

Structural origins of FRET-observed nascent chain compaction on the ribosome

Abstract:

A fluorescence signal arising from a Förster resonance energy transfer process was used to monitor conformational changes of a domain within the E. coli protein HemK during its synthesis by the ribosome. An increase in fluorescence was observed to begin 10 s after translation was initiated, indicating the domain became more compact in size. Since fluorescence only reports a single value at each time point it contains very little information about the structural ensemble that gives rise to it....

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1021/acs.jpcb.8b07726

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Division:
MPLS
Department:
Statistics
Role:
Author
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Role:
Author
ORCID:
0000-0001-9809-3273
Publisher:
American Chemical Society Publisher's website
Journal:
Journal of Physical Chemistry B Journal website
Volume:
122
Issue:
43
Pages:
9927-9937
Publication date:
2018-09-28
Acceptance date:
2018-09-19
DOI:
EISSN:
1520-5207
ISSN:
1520-6106
Pmid:
30265800

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