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Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry

Abstract:

Membrane proteins reside in lipid bilayers and are typically extracted from this environment for study, which often compromises their integrity. In this work, we ejected intact assemblies from membranes, without chemical disruption, and used mass spectrometry to define their composition. From Escherichia coli outer membranes, we identified a chaperone-porin association and lipid interactions in the β-barrel assembly machinery. We observed efflux pumps bridging inner and outer membranes, and f...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1126/science.aau0976

Authors


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Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
ORCID:
0000-0002-0751-0092
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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Sub department:
Structural Biology
Role:
Author
ORCID:
0000-0001-9578-4263
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry
Role:
Author
More by this author
Role:
Author
ORCID:
0000-0002-7115-1565
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Wellcome Trust More from this funder
European Research Council More from this funder
Publisher:
American Association for the Advancement of Science Publisher's website
Journal:
Science Journal website
Volume:
362
Issue:
6416
Pages:
829-834
Publication date:
2018-11-16
Acceptance date:
2018-10-08
DOI:
EISSN:
1095-9203
ISSN:
0036-8075
Pmid:
30442809
Source identifiers:
945352
Language:
English
Pubs id:
pubs:945352
UUID:
uuid:1c628ad7-e4c9-4ba7-b198-9a12d7bcd405
Local pid:
pubs:945352
Deposit date:
2019-01-22

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