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Experimental generation and computational modeling of intracellular pH gradients in cardiac myocytes.

Abstract:

It is often assumed that pH(i) is spatially uniform within cells. A double-barreled microperfusion system was used to apply solutions of weak acid (acetic acid, CO(2)) or base (ammonia) to localized regions of an isolated ventricular myocyte (guinea pig). A stable, longitudinal pH(i) gradient (up to 1 pH(i) unit) was observed (using confocal imaging of SNARF-1 fluorescence). Changing the fractional exposure of the cell to weak acid/base altered the gradient, as did changing the concentration ...

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Publication status:
Published

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Publisher copy:
10.1529/biophysj.104.051391

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Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Role:
Author
Journal:
Biophysical journal
Volume:
88
Issue:
4
Pages:
3018-3037
Publication date:
2005-04-01
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
Source identifiers:
106296

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