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Thesis

Applications of interferometric scattering microscopy (iSCAT) to single particle tracking in model and cell membranes

Abstract:

We have employed Interferometric Scattering (iSCAT) microscopy-based Single Particle Tracking (SPT) to study lipid dynamics of on model membranes and plasma membranes. This novel imaging technique offers high localization precision (~ 101 nm) and temporal resolution (< msec), together with tunable Signal-to-Noise Ratio.

In the Materials and Methods, we describe our custom iSCAT setup. Also, an advanced analysis pipeline for SPT data is introduced, to estimate camera b...

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Division:
MSD
Department:
Biomedical Services
Role:
Author

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Supervisor
Engineering & Physical Sciences Research Council More from this funder
Type of award:
DPhil
Level of award:
Doctoral
Awarding institution:
University of Oxford

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