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Thesis

Synthetic phosphorylation of kinases for functional studies in vitro

Abstract:

The activity of protein kinases is heavily dependent on the phosphorylation state of the protein. Kinase phosphorylation states have been prepared through biological or enzymatic means for biochemical evaluation, but the use of protein chemical modification as an investigative tool has not been addressed. By chemically reacting a genetically encoded cysteine, phosphocysteine was installed via dehydroalanine as a reactive intermediate. The installed phosphocysteine was intended as a surroga...

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author

Contributors

Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Chemistry Research Laboratory
Role:
Supervisor
Department:
Chemical Biology Group, BioTherapeutics Chemistry, WorldWide Medicinal Chemistry, Pfizer, 200 Cambridge Park Drive, Cambridge Massachusetts 01890, United States
Role:
Supervisor
Engineering and Physical Sciences Research Council More from this funder
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Grant:
DM4920 SOF S4731
Type of award:
DPhil
Level of award:
Doctoral
Awarding institution:
University of Oxford

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