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An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography

Abstract:

Serial femtosecond crystallography has opened up many new opportunities in structural biology. In recent years, several approaches employing light-inducible systems have emerged to enable time-resolved experiments that reveal protein dynamics at high atomic and temporal resolutions. However, very few enzymes are light-dependent, whereas macromolecules requiring ligand diffusion into an active site are ubiquitous. In this work we present a drop-on-drop sample delivery system that enables the s...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41467-021-24757-7

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Role:
Author
ORCID:
0000-0002-5227-4770
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Role:
Author
ORCID:
0000-0002-2859-4475
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Role:
Author
ORCID:
0000-0002-1793-7030
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Publisher:
Springer Nature Publisher's website
Journal:
Nature Communications Journal website
Volume:
12
Issue:
1
Article number:
4461
Publication date:
2021-07-22
Acceptance date:
2021-07-01
DOI:
EISSN:
2041-1723
Pmid:
34294694

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