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Journal article

Precise tuning of gene expression levels in mammalian cells

Abstract:

Precise, analogue regulation of gene expression is critical for cellular function in mammals. In contrast, widely employed experimental and therapeutic approaches such as knock-in/out strategies are more suitable for binary control of gene activity. Here we report on a method for precise control of gene expression levels in mammalian cells using engineered microRNA response elements (MREs). First, we measure the efficacy of thousands of synthetic MRE variants under the control of an endogenou...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41467-019-08777-y

Authors


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Institution:
University of Oxford
Department:
RDM
Role:
Author
ORCID:
0000-0002-6857-5922
More by this author
Institution:
University of Oxford
Department:
RDM
Role:
Author
ORCID:
0000-0002-5892-1174
More by this author
Institution:
University of Oxford
Department:
RDM
Role:
Author
More by this author
Institution:
University of Oxford
Department:
RDM
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Biochemistry
Role:
Author
ORCID:
0000-0002-4947-6048
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Publisher:
Springer Nature Publisher's website
Journal:
Nature Communications Journal website
Volume:
10
Article number:
818
Publication date:
2019-02-18
Acceptance date:
2019-01-29
DOI:
EISSN:
2041-1723
Pmid:
30778069
Source identifiers:
975010
Language:
English
Pubs id:
pubs:975010
UUID:
uuid:2bdd670e-95be-4e3d-8aa7-4409dba88923
Local pid:
pubs:975010
Deposit date:
2019-07-31

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