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Population dynamics and gene transfer in genetically modified bacteria in a model microcosm.

Abstract:

The horizontal transfer and effects on host fitness of a neutral gene cassette inserted into three different genomic loci of a plant-colonizing pseudomonad was assessed in a model ecosystem. The KX reporter cassette (kanamycin resistance, aph, and catechol 2, 3, dioxygenase, xylE) was introduced on the disarmed transposon mini-Tn5 into: (I) the chromosome of a spontaneous rifampicin resistant mutant Pseudomonas fluorescens SBW25R; (II) the chromosome of SBW25R in the presence of a naturally o...

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Publication status:
Published

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Journal:
Molecular ecology
Volume:
12
Issue:
11
Pages:
3097-3107
Publication date:
2003-11-01
DOI:
EISSN:
1365-294X
ISSN:
0962-1083
Source identifiers:
210519

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