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Structure of the cyanobactin oxidase ThcOx from Cyanothece sp. PCC 7425, the first structure to be solved at Diamond Light Source beamline I23 by means of S-SAD.

Abstract:

Determination of protein crystal structures requires that the phases are derived independently of the observed measurement of diffraction intensities. Many techniques have been developed to obtain phases, including heavy-atom substitution, molecular replacement and substitution during protein expression of the amino acid methionine with selenomethionine. Although the use of selenium-containing methionine has transformed the experimental determination of phases it is not always possible, eithe...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1107/S2059798316015850

Authors


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Royal Society More from this funder
Sichuan University More from this funder
Publisher:
International Union of Crystallography Publisher's website
Journal:
Acta Crystallographica Section D: Structural Biology Journal website
Volume:
72
Issue:
Pt 11
Pages:
1174-1180
Publication date:
2017-10-01
Acceptance date:
2016-10-07
DOI:
ISSN:
2059-7983
Source identifiers:
699009
Language:
English
Keywords:
Pubs id:
pubs:699009
UUID:
uuid:36986752-fbc0-4338-8505-9c9fcf0c662a
Local pid:
pubs:699009
Deposit date:
2017-07-13

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