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Journal article

Genetic incorporation of olefin cross-metathesis reaction tags for protein modification

Abstract:

Olefin cross-metathesis (CM) is a viable reaction for the modification of alkene-containing proteins. Although allyl sulfide or selenide side-chain motifs in proteins can critically enhance the rate of CM reactions, no efficient method for their site-selective genetic incorporation into proteins has been reported to date. Here, through the systematic evaluation of olefin-bearing unnatural amino acids for their metabolic incorporation, we have discovered S-allylhomocysteine (Ahc) as a genetica...

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Publication status:
Published
Peer review status:
Peer reviewed

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Files:
Publisher copy:
10.1021/jacs.8b09433

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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDORMS
Sub department:
KIR
Role:
Author
More from this funder
Funding agency for:
Davis, B
Grant:
Wolfson Research Merit Award
More from this funder
Funding agency for:
Bhushan, B
Cancer Research UK More from this funder
Engineering and Physical Sciences Research Council More from this funder
Publisher:
American Chemical Society Publisher's website
Journal:
Journal of the American Chemical Society Journal website
Volume:
140
Issue:
44
Pages:
14599-14603
Publication date:
2018-10-16
Acceptance date:
2018-10-16
DOI:
EISSN:
1520-5126
ISSN:
0002-7863
Pmid:
30371070
Language:
English
Pubs id:
pubs:935835
UUID:
uuid:37436f1d-1c5b-46f1-8a9f-a295d54677ce
Local pid:
pubs:935835
Source identifiers:
935835
Deposit date:
2018-12-16

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