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SpyRings declassified: a blueprint for using isopeptide-mediated cyclization to enhance enzyme thermal resilience

Abstract:

Enzymes often have marginal stability, with unfolding typically leading to irreversible denaturation. This sensitivity is a major barrier, both for de novo enzyme development and for expanding enzyme impact beyond the laboratory. Seeking an approach to enhance resilience to denaturation that could be applied to a range of different enzymes, we developed SpyRing cyclization. SpyRings contain genetically encoded SpyTag (13 amino acids) on the N-terminus and SpyCatcher (12 kDa) on the C-terminus...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/bs.mie.2016.05.004

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
Publisher:
Elsevier Publisher's website
Journal:
Methods in Enzymology Journal website
Publication date:
2016-01-01
Acceptance date:
2016-05-25
DOI:
EISSN:
1557-7988
ISSN:
0076-6879
Source identifiers:
631611
Keywords:
Pubs id:
pubs:631611
UUID:
uuid:374c4b66-cb20-40a5-a3c8-7e5ce67f9dff
Local pid:
pubs:631611
Deposit date:
2016-07-02

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