Journal article
SpyRings declassified: a blueprint for using isopeptide-mediated cyclization to enhance enzyme thermal resilience
- Abstract:
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Enzymes often have marginal stability, with unfolding typically leading to irreversible denaturation. This sensitivity is a major barrier, both for de novo enzyme development and for expanding enzyme impact beyond the laboratory. Seeking an approach to enhance resilience to denaturation that could be applied to a range of different enzymes, we developed SpyRing cyclization. SpyRings contain genetically encoded SpyTag (13 amino acids) on the N-terminus and SpyCatcher (12 kDa) on the C-terminus...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Authors
Funding
+ Biotechnology and Biological Sciences Research Council
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Funding agency for:
Schoene, C
Bibliographic Details
- Publisher:
- Elsevier Publisher's website
- Journal:
- Methods in Enzymology Journal website
- Publication date:
- 2016-01-01
- Acceptance date:
- 2016-05-25
- DOI:
- EISSN:
-
1557-7988
- ISSN:
-
0076-6879
Item Description
- Keywords:
- Pubs id:
-
pubs:631611
- UUID:
-
uuid:374c4b66-cb20-40a5-a3c8-7e5ce67f9dff
- Local pid:
- pubs:631611
- Source identifiers:
-
631611
- Deposit date:
- 2016-07-02
Terms of use
- Copyright holder:
- Elsevier
- Copyright date:
- 2016
- Notes:
- Copyright © 2016 Elsevier Inc. All rights reserved.
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