Individual Timp deficiencies differentially impact pro-MMP-2 activation.

Journal article

Membrane-type matrix metalloproteinases (MT-MMPs) have emerged as key enzymes in tumor cell biology. The importance of MT1-MMP, in particular, is highlighted by its ability to activate pro-MMP-2 at the cell surface through the formation of a trimolecular complex comprised of MT1-MMP/tissue inhibitor of metalloproteinase-2 (TIMP-2)/pro-MMP-2. TIMPs 1-4 are physiological MMP inhibitors with distinct roles in the regulation of pro-MMP-2 processing. Here, we have shown that individual Timp defici...

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Authors: English, J; Kassiri, Z; Koskivirta, I; Atkinson, S; Di Grappa, M

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 281
• Issue: 15
• Pages: 10337-10346
• Publication date: 2006-04-01
• DOI: 10.1074/jbc.m512009200
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Models, Biological; Time Factors; Humans; Electrophoresis, Polyacrylamide Gel; RNA, Messenger; Tissue Inhibitor of Metalloproteinases; Recombinant Proteins; Mice, Transgenic; Animals; Crosses, Genetic; Cytochalasin D; Cell Line; Enzyme Activation; Mice, Inbred C57BL; Blotting, Western; Reverse Transcriptase Polymerase Chain Reaction; Proteins; Concanavalin A; Matrix Metalloproteinase 2; Matrix Metalloproteinases; Embryo, Mammalian; Cricetinae; Mice; Fibroblasts; Matrix Metalloproteinase 15; Matrix Metalloproteinases, Membrane-Associated; Tissue Inhibitor of Metalloproteinase-2; Protein Binding; Tissue Inhibitor of Metalloproteinase-3; Matrix Metalloproteinase 14; Matrix Metalloproteinase 16