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An improved method for quantitative ChIP studies of nuclear receptor function

Abstract:

Chromatin immunoprecipitation (ChIP) is a valuable tool for the endocrine researcher, providing a means to measure the recruitment of hormone-activated nuclear receptors, for example. However, the technique can be challenging to perform and has multiple experimental steps, risking introduction of error at each. The data produced can be challenging to interpret; several different methods are commonly used for normalising data and thus comparing between conditions. Absolute, sensitive quantific...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1530/jme-18-0243

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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Sub department:
OCDEM
Department:
Unknown
Role:
Author
Publisher:
Bioscientifica Publisher's website
Journal:
Journal of Molecular Endocrinology Journal website
Volume:
62
Issue:
4
Pages:
169-177
Publication date:
2019-05-01
Acceptance date:
2019-03-11
DOI:
EISSN:
1479-6813
ISSN:
0952-5041
Source identifiers:
985608
Keywords:
Pubs id:
pubs:985608
UUID:
uuid:37cdc20e-d0b5-48e5-976b-e49acbfcbb0b
Local pid:
pubs:985608
Deposit date:
2019-03-30

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