Journal article
Wavelength considerations in confocal microscopy of botanical specimens
- Abstract:
-
We have used a multiple-laser confocal microscope with lines 325, 442, 488, 514 and 633 nm to investigate optical sectioning of botanical specimens over a wide range of wavelengths. The 442-nm line allowed efficient excitation of Chromomycin A3, with minimal background autofluorescence, to visualize GC-rich heterochromatin as an aid to chromosome identification. Sequential excitation with 442- and 488-nm light enabled ratio imaging of cytosolic pH using BCECF. The red HeNe laser penetrated de...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Authors
Funding
Bibliographic Details
- Publisher:
- Wiley Publisher's website
- Journal:
- Journal of Microscopy Journal website
- Volume:
- 166
- Issue:
- 1
- Pages:
- 29-42
- Publication date:
- 1992-04-01
- DOI:
- ISSN:
-
0022-2720
- Source identifiers:
-
43780
Item Description
- Keywords:
- Pubs id:
-
pubs:43780
- UUID:
-
uuid:38d0a39d-caf8-4e6b-8a84-faa96ee58ddd
- Local pid:
- pubs:43780
- Deposit date:
- 2012-12-19
Terms of use
- Copyright holder:
- The Royal Microscopical Society
- Copyright date:
- 1992
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