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Evolutionary repurposing of a sulfatase: A new Michaelis complex leads to efficient transition state charge offset

Abstract:

The recruitment and evolutionary optimization of promiscuous enzymes is key to the rapid adaptation of organisms to changing environments. Our understanding of the precise mechanisms underlying enzyme repurposing is, however, limited: What are the active-site features that enable the molecular recognition of multiple substrates with contrasting catalytic requirements? To gain insights into the molecular determinants of adaptation in promiscuous enzymes, we performed the laboratory evolutio...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1073/pnas.1607817115

Authors


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Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry
Sub department:
Organic Chemistry
Department:
Unknown
Role:
Author
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Biotechnology and Biological Sciences Research Council More from this funder
Engineering and Physical Sciences Research Council More from this funder
Publisher:
National Academy of Sciences Publisher's website
Journal:
Proceedings of the National Academy of Sciences Journal website
Volume:
115
Issue:
31
Pages:
E7293-E7302
Publication date:
2018-07-16
Acceptance date:
2018-05-31
DOI:
EISSN:
1091-6490
ISSN:
0027-8424
Source identifiers:
974247
Language:
English
Keywords:
Pubs id:
pubs:974247
UUID:
uuid:3918a8f6-5959-4e9c-930b-4360f2c0042a
Local pid:
pubs:974247
Deposit date:
2019-07-23

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