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Journal article

Single-molecule imaging of electroporated dye-labelled CheY in live E. coli

Abstract:

For the last two decades, the use of genetically fused Fluorescent Proteins has greatly contributed to the study of chemotactic signalling in E. coli including the activation of the response regulator protein CheY and its interaction with the flagellar motor. However, this approach suffers from a number of limitations, both biological and biophysical: for example, not all fusions are fully functional when fused to a bulky FP, which can have a ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1098/rstb.2015.0492

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Sub department:
Condensed Matter Physics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
More from this funder
Funding agency for:
Di Paolo, D
Grant:
DTC studentship
Biotechnology and Biological Sciences Research Council More from this funder
Publisher:
Royal Society Publisher's website
Journal:
Philosophical Transactions B Journal website
Volume:
371
Issue:
1707
Publication date:
2016-09-26
Acceptance date:
2016-07-02
DOI:
Source identifiers:
633471
Keywords:
Pubs id:
pubs:633471
UUID:
uuid:3ce73779-6b1f-4726-9664-c4c01dc26206
Local pid:
pubs:633471
Deposit date:
2016-09-07

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