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DySCo: Quantitating associations of membrane proteins using two-color single-molecule tracking

Abstract:
We present a general method called dynamic single-molecule colocalization for quantitating the associations of single cell surface molecules labeled with distinct autofluorescent proteins. The chief advantages of the new quantitative approach are that, in addition to stable interactions, it is capable of measuring nonconstitutive associations, such as those induced by the cytoskeleton, and it is applicable to situations where the number of molecules is small. © 2009 by the Biophysical Society.

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Publisher copy:
10.1016/j.bpj.2009.05.046

Authors


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Journal:
Biophysical Journal
Volume:
97
Issue:
4
Publication date:
2009-08-19
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
Source identifiers:
94570
Language:
English
Pubs id:
pubs:94570
UUID:
uuid:3cedb359-8472-4d6c-b23c-1ba593aae848
Local pid:
pubs:94570
Deposit date:
2012-12-19

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