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Journal article

The different effects of substrates and nucleotides on the complex formation of ABC transporters

Abstract:

The molybdate importer (ModBC-A of Archaeoglobus fulgidus) and the vitamin B12 importer (BtuCD-F of Escherichia coli) are members of the type I and type II ABC importer families. Here we study the influence of substrate and nucleotide binding on complex formation and stability. Using native mass spectrometry we show that the interaction between the periplasmic substrate-binding protein (SBP) ModA and the transporter ModBC is dependent upon binding of molybdate. By contrast, vitamin B12 disrup...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.str.2019.01.010

Authors


More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry
Sub department:
Physical and Theoretical Chemistry Laboratory
Role:
Author
ORCID:
0000-0003-4346-182X
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Oxford college:
Exeter College
Role:
Author
Publisher:
Elsevier Publisher's website
Journal:
Structure Journal website
Volume:
27
Issue:
4
Pages:
651-659
Publication date:
2019-02-21
Acceptance date:
2019-01-18
DOI:
ISSN:
0969-2126 and 1878-4186
Pmid:
30799075
Source identifiers:
980868
Language:
English
Keywords:
Pubs id:
pubs:980868
UUID:
uuid:3cfba2d6-09cf-4f94-b3d5-dc82adc3e2f7
Local pid:
pubs:980868
Deposit date:
2019-04-08

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