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Journal article

Super-resolution single molecule fish at the drosophila neuromuscular junction

Abstract:

The lack of an effective, simple, and highly sensitive protocol for fluorescent in situ hybridization (FISH) at the Drosophila larval neuromuscular junction (NMJ) has hampered the study of mRNA biology. Here, we describe our modified single molecule FISH (smFISH) methods that work well in whole mount Drosophila NMJ preparations to quantify primary transcription and count individual cytoplasmic mRNA molecules in specimens while maintaining ultrastructural prese...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1007/978-1-4939-7213-5_10

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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Oxford college:
Jesus College
Role:
Author
Publisher:
Springer Publisher's website
Journal:
Methods in Molecular Biology Journal website
Volume:
1649
Pages:
163-175
Publication date:
2018-01-01
Acceptance date:
2017-09-14
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
Pmid:
29450865
Source identifiers:
826082
Language:
English
Keywords:
Pubs id:
pubs:826082
UUID:
uuid:3f873bbd-0508-428c-bb81-129af63bc68c
Local pid:
pubs:826082
Deposit date:
2019-02-27

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