Journal article
Super-resolution single molecule fish at the drosophila neuromuscular junction
- Abstract:
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The lack of an effective, simple, and highly sensitive protocol for fluorescent in situ hybridization (FISH) at the Drosophila larval neuromuscular junction (NMJ) has hampered the study of mRNA biology. Here, we describe our modified single molecule FISH (smFISH) methods that work well in whole mount Drosophila NMJ preparations to quantify primary transcription and count individual cytoplasmic mRNA molecules in specimens while maintaining ultrastructural prese...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Version of record, pdf, 12.8MB)
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- Publisher copy:
- 10.1007/978-1-4939-7213-5_10
Authors
Bibliographic Details
- Publisher:
- Springer Publisher's website
- Journal:
- Methods in Molecular Biology Journal website
- Volume:
- 1649
- Pages:
- 163-175
- Publication date:
- 2018-01-01
- Acceptance date:
- 2017-09-14
- DOI:
- EISSN:
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1940-6029
- ISSN:
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1064-3745
- Pmid:
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29450865
- Source identifiers:
-
826082
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:826082
- UUID:
-
uuid:3f873bbd-0508-428c-bb81-129af63bc68c
- Local pid:
- pubs:826082
- Deposit date:
- 2019-02-27
Terms of use
- Copyright holder:
- Titlow et al
- Copyright date:
- 2018
- Notes:
- © The Authors 2018. This chapter is licensed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.
- Licence:
- CC Attribution (CC BY)
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