Journal article icon

Journal article

Simultaneous single-molecule epigenetic imaging of DNA methylation and hydroxymethylation

Abstract:

The modifications 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) are the two major DNA epigenetic modifications in mammalian genomes and play crucial roles in development and pathogenesis. Little is known about the colocalization or potential correlation of these two modifications. Here we present an ultrasensitive single-molecule imaging technology capable of detecting and quantifying 5hmC and 5mC from trace amounts of DNA. We used this approach to perform single-molecule fluoresc...

Expand abstract
Publication status:
Published
Peer review status:
Peer reviewed

Actions


Access Document


Publisher copy:
10.1073/pnas.1600223113

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Oxford Ludwig Institute
Role:
Author
Publisher:
National Academy of Sciences Publisher's website
Journal:
Proceedings of the National Academy of Sciences of the United States of America Journal website
Volume:
113
Issue:
16
Pages:
4338–4343
Publication date:
2016-04-19
Acceptance date:
2016-03-28
DOI:
EISSN:
1091-6490
ISSN:
0027-8424
Source identifiers:
628429
Language:
English
Keywords:
Pubs id:
pubs:628429
UUID:
uuid:3fa4ed9e-2467-4c13-a58b-a73214ed8a56
Local pid:
pubs:628429
Deposit date:
2016-06-17

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP