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Journal article

Robust detection of chromosomal interactions from small numbers of cells using low-input Capture-C

Abstract:

Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This hampers the investigation of chromatin architecture in rare cell populations. We present a new low-input Capture-C approach that can generate high-quality 3C interaction profiles from 10 000-20 000 cells, depending on the resolution used for analysis. We also present a PCR-free, sequencing-free 3C techniq...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1093/nar/gkx1194

Authors


More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM; Weatherall Insti. of Molecular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
RDM Clinical Laboratory Sciences
Role:
Author
ORCID:
0000-0002-4108-4357
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM; RDM Clinical Laboratory Sciences
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM; Weatherall Insti. of Molecular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM; RDM Clinical Laboratory Sciences
Role:
Author
Publisher:
Oxford University Press Publisher's website
Journal:
Nucleic Acids Research Journal website
Volume:
45
Issue:
22
Article number:
e184
Publication date:
2017-11-23
Acceptance date:
2017-11-21
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
Pmid:
29186505
Source identifiers:
809379
Language:
English
Keywords:
Pubs id:
pubs:809379
UUID:
uuid:404e1171-3977-457a-a305-5afd4c4bf4c8
Local pid:
pubs:809379
Deposit date:
2018-05-11

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