Residual dipolar couplings: are multiple independent alignments always possible?
RDCs for the 14 kDa protein hen egg-white lysozyme (HEWL) have been measured in eight different alignment media. The elongated shape and strongly positively charged surface of HEWL appear to limit the protein to four main alignment orientations. Furthermore, low levels of alignment and the protein's interaction with some alignment media increases the experimental error. Together with heterogeneity across the alignment media arising from constraints on temperature, pH and ionic strength for so...Expand abstract
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- V A Higman et al
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- Citation: Higman, V. A. et al. (2011). 'Residual dipolar couplings: are multiple independent alignments always possible?', Journal of Biomolecular NMR 49(1), 53-60 [Available at http://www.springer.com/physics/biophysics+%26+biological+physics/journal/10858]. © the authors 2010. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original authors and source are credited.
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