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Journal article

FRET biosensor uncovers cAMP nano-domains at β-adrenergic targets that dictate precise tuning of cardiac contractility

Abstract:

Compartmentalized cAMP/PKA signalling is now recognized as important for physiology and pathophysiology, yet a detailed understanding of the properties, regulation and function of local cAMP/PKA signals is lacking. Here we present a fluorescence resonance energy transfer (FRET)-based sensor, CUTie, which detects compartmentalized cAMP with unprecedented accuracy. CUTie, targeted to specific multiprotein complexes at discrete plasmalemmal, sarcoplasmic reticular and myofilament sites, reveals ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/ncomms15031

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Role:
Author
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Funding agency for:
Zaccolo, M
Grant:
RE/08/004
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Grant:
PG/10/75/28537,RG/12/3/29423
PG/15/5/31110
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Publisher:
Springer Nature Publisher's website
Journal:
Nature Communications Journal website
Volume:
8
Article number:
15031
Publication date:
2017-04-20
Acceptance date:
2017-02-21
DOI:
ISSN:
2041-1723
Source identifiers:
690289
Pubs id:
pubs:690289
UUID:
uuid:42e472d0-cfff-4024-963c-08a93bf7fcf5
Local pid:
pubs:690289
Deposit date:
2017-04-20

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