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Journal article

High-speed multifocal plane fluorescence microscopy for three-dimensional visualisation of beating flagella.

Abstract:

Analysis of flagellum and cilium beating in three dimensions (3D) is important for understanding cell motility, and using fluorescence microscopy to do so would be extremely powerful. Here, high-speed multifocal plane fluorescence microscopy, where the light path is split to visualise multiple focal planes simultaneously, was used to reconstruct Trypanosoma brucei and Leishmania mexicana movement in 3D. These species are uniflagellate unicellular parasites for which motility is vital. It was ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1242/jcs.231795

Authors


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Institution:
University of Oxford
Division:
MPLS Division
Department:
Mathematical Institute
Sub department:
Biochemistry
Oxford college:
Wadham College
Role:
Author
ORCID:
0000-0003-0853-267X
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
ORCID:
0000-0002-4270-8360
Publisher:
Company of Biologists Publisher's website
Journal:
Journal of Cell Science Journal website
Volume:
132
Issue:
16
Pages:
jcs231795-jcs231795
Publication date:
2019-08-15
Acceptance date:
2019-07-22
DOI:
EISSN:
1477-9137
ISSN:
0021-9533
Pmid:
31371486
Source identifiers:
1038819
Language:
English
Keywords:
Pubs id:
pubs:1038819
UUID:
uuid:4e8f76eb-56d0-4ec0-a529-ee9845e6a7e8
Local pid:
pubs:1038819
Deposit date:
2019-08-22

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