Book section
A bioluminescent Agrobacterium tumefaciens for imaging bacterial metabolic activity in planta
- Abstract:
- Bioluminescence enables the monitoring of spatiotemporal dynamics and activity of bacterial populations in planta. We here describe a procedure to use AgroLux, a bioluminescent Agrobacterium tumefaciens, as a tool to study bacterial responses upon agroinfiltration. The first method details how to transform bioluminescent AgroLux to carry binary plasmids of interests. Then, a simple agroinfiltration assay for in planta imaging of bioluminescence signals is presented. AgroLux assays will increase our understanding of plant-Agrobacterium interactions and plant immunity and improve molecular farming.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Access Document
- Files:
-
-
(Preview, Accepted manuscript, pdf, 219.2KB, Terms of use)
-
- Publisher copy:
- 10.1007/978-1-0716-2241-4_15
Authors
+ European Commission
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- Funder identifier:
- https://ror.org/00k4n6c32
- Grant:
- 760331
+ European Research Council
More from this funder
- Funder identifier:
- https://ror.org/0472cxd90
- Grant:
- 616449
+ Biotechnology and Biological Sciences Research Council
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- Funder identifier:
- https://ror.org/00cwqg982
- Publisher:
- Springer
- Pages:
- 285-293
- Series:
- Methods in Molecular Biology
- Series number:
- 2480
- Place of publication:
- United States
- Publication date:
- 2022-05-27
- DOI:
- EISSN:
-
1940-6029
- ISSN:
-
1064-3745
- Pmid:
-
35616868
- EISBN:
- 9781071622414
- ISBN:
- 9781071622407
- Language:
-
English
- Keywords:
- Pubs id:
-
1261357
- Local pid:
-
pubs:1261357
- Deposit date:
-
2025-04-29
Terms of use
- Copyright holder:
- Jutras et al.
- Copyright date:
- 2022
- Rights statement:
- © 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
- Notes:
- This is the accepted manuscript version of the article. The final version is available online from Springer at https://dx.doi.org/10.1007/978-1-0716-2241-4_15
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