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Quantifying distortions in two-photon remote focussing microscope images using a volumetric calibration specimen

Abstract:

Remote focussing microscopy allows sharp, in-focus images to be acquired at high speed from outside of the focal plane of an objective lens without any agitation of the specimen. However, without careful optical alignment, the advantages of remote focussing microscopy could be compromised by the introduction of depth-dependent scaling artifacts. To achieve an ideal alignment in a point-scanning remote focussing microscope, the lateral (XY) scan mirror pair must be imaged onto the back focal p...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.3389/fphys.2014.00384

Authors


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Role:
Author
ORCID:
0000-0003-1645-5475
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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pharmacology
Role:
Author
ORCID:
0000-0002-0904-3862
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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Physiology Anatomy and Genetics
Role:
Author
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Institution:
University of Oxford
Division:
MPLS Division
Department:
Engineering Science
Oxford college:
New College
Role:
Author
ORCID:
0000-0003-1688-259X
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Physiology Anatomy and Genetics
Role:
Author
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Publisher:
Frontiers Media
Journal:
Frontiers in physiology Journal website
Issue:
OCT
Publication date:
2014-01-01
Acceptance date:
2014-09-18
DOI:
ISSN:
1664-042X
Pmid:
25339910
Source identifiers:
487415
Language:
English
Keywords:
Pubs id:
pubs:487415
UUID:
uuid:4fab6559-502b-4a12-a02d-adddb14a3af1
Local pid:
pubs:487415
Deposit date:
2019-05-08

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