Journal article
Quantifying distortions in two-photon remote focussing microscope images using a volumetric calibration specimen
- Abstract:
-
Remote focussing microscopy allows sharp, in-focus images to be acquired at high speed from outside of the focal plane of an objective lens without any agitation of the specimen. However, without careful optical alignment, the advantages of remote focussing microscopy could be compromised by the introduction of depth-dependent scaling artifacts. To achieve an ideal alignment in a point-scanning remote focussing microscope, the lateral (XY) scan mirror pair must be imaged onto the back focal p...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Funding
Bibliographic Details
- Publisher:
- Frontiers Media
- Journal:
- Frontiers in physiology Journal website
- Issue:
- OCT
- Publication date:
- 2014-01-01
- Acceptance date:
- 2014-09-18
- DOI:
- ISSN:
-
1664-042X
- Pmid:
-
25339910
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:487415
- UUID:
-
uuid:4fab6559-502b-4a12-a02d-adddb14a3af1
- Local pid:
- pubs:487415
- Source identifiers:
-
487415
- Deposit date:
- 2019-05-08
Terms of use
- Copyright holder:
- Alexander D Corbett et al
- Copyright date:
- 2014
- Notes:
- © 2014 Corbett, Burton, Bub, Salter, Tuohy, Booth and Wilson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.>
- Licence:
- CC Attribution (CC BY)
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