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Native desorption electrospray ionization liberates soluble and membrane protein complexes from surfaces

Abstract:

Mass spectrometry (MS) applications for intact protein complexes typically require electrospray (ES) ionization and have not been achieved via direct desorption from surfaces. Desorption ES ionization (DESI) MS has however transformed the study of tissue surfaces through release and characterisation of small molecules. Motivated by the desire to screen for ligand binding to intact protein complexes we report the development of a native DESI platform. By establishing conditions that preserve n...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1002/anie.201704849

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
More from this funder
Funding agency for:
Robinson, C
Grant:
Investigator Award (104633/Z/14/Z)
More from this funder
Funding agency for:
Robinson, C
Grant:
Investigator Award (104633/Z/14/Z)
More from this funder
Funding agency for:
Robinson, C
Grant:
Investigator Award (104633/Z/14/Z)
Publisher:
Wiley Publisher's website
Journal:
Angewandte Chemie International Edition Journal website
Volume:
56
Issue:
46
Pages:
14463–14468
Publication date:
2017-09-18
Acceptance date:
2017-09-06
DOI:
EISSN:
1521-3773
ISSN:
1433-7851
Pmid:
28884954
Language:
English
Keywords:
Pubs id:
pubs:730106
UUID:
uuid:52bb3fae-7c47-484a-85bc-500e72be2ee5
Local pid:
pubs:730106
Source identifiers:
730106
Deposit date:
2017-09-22

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