Native desorption electrospray ionization liberates soluble and membrane protein complexes from surfaces
Mass spectrometry (MS) applications for intact protein complexes typically require electrospray (ES) ionization and have not been achieved via direct desorption from surfaces. Desorption ES ionization (DESI) MS has however transformed the study of tissue surfaces through release and characterisation of small molecules. Motivated by the desire to screen for ligand binding to intact protein complexes we report the development of a native DESI platform. By establishing conditions that preserve n...Expand abstract
- Publication status:
- Peer review status:
- Peer reviewed
(Version of record, pdf, 4.3MB)
- Publisher copy:
- Copyright holder:
- Ambrose et al
- Copyright date:
Copyright © 2017 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- CC Attribution (CC BY)
If you are the owner of this record, you can report an update to it here: Report update to this record