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Journal article

Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation.

Abstract:

Background CRISPR-Cas9 gene-editing technology has facilitated the generation of knockout mice, providing an alternative to cumbersome and time-consuming traditional embryonic stem cell-based methods. An earlier study reported up to 16% efficiency in generating conditional knockout (cKO or floxed) alleles by microinjection of 2 single guide RNAs (sgRNA) and 2 single-stranded oligonucleotides as donors (referred herein as “two-donor floxing” method).

Result

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1186/s13059-019-1776-2

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Role:
Author
ORCID:
0000-0002-8022-4033
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Publisher:
BioMed Central Publisher's website
Journal:
Genome Biology Journal website
Volume:
20
Issue:
1
Pages:
171
Publication date:
2019-08-26
Acceptance date:
2019-07-27
DOI:
EISSN:
1474-760X
ISSN:
1474-7596
Pmid:
31446895
Source identifiers:
1048711
Language:
English
Keywords:
Pubs id:
pubs:1048711
UUID:
uuid:53c52dbc-5d57-43e5-b0f6-9e3afb3a627b
Local pid:
pubs:1048711
Deposit date:
2019-09-05

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