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Thesis

Engineered α-hemolysin pores with chemically and genetically-fused functional proteins

Abstract:

Protein engineering could be used to bring two proteins together, which don't normally interact, in an oriented configuration. Using computer modelling and experimental work involving mutagenesis, a new dimer complex, (α7)2, was engineered with two α-hemolysin (αHL) heptamers (α7) units linked via disulfide bridges in a cap-to-cap orientation. The structure of (α7)2 was confirmed by biochemical analysis, transmission electron microscopy (TEM) and single-channel electrical recording. Import...

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Chemical Biology
Oxford college:
St Cross College
Role:
Author

Contributors

Division:
MPLS
Department:
Chemistry
Sub department:
Chemical Biology
Role:
Supervisor
Publication date:
2013
Type of award:
DPhil
Level of award:
Doctoral
Awarding institution:
Oxford University, UK
Language:
English
Subjects:
UUID:
uuid:55450bd3-b93f-410f-b795-0110449c0da9
Local pid:
ora:7144
Deposit date:
2013-08-07

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