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Journal article

Orientation of the OmpF porin in planar lipid bilayers

Abstract:

The outer-membrane protein OmpF is an abundant trimeric general diffusion porin that plays a central role in the transport of antibiotics and colicins across the outer membrane of E. coli. Individual OmpF trimers in planar lipid bilayers (PLBs) show one of two current–voltage asymmetries, thus implying that insertion occurs with either the periplasmic or the extracellular end first. A method for establishing the orientation of OmpF in PLB was developed, based on targeted covalent modification...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1002/cbic.201600644

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Institution:
University of Oxford
Oxford college:
Lincoln College
Role:
Author
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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Chemistry Research Laboratory
Role:
Author
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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
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Publisher:
Wiley Publisher's website
Journal:
ChemBioChem
Volume:
18
Issue:
6
Pages:
554-562
Publication date:
2017-02-28
Acceptance date:
2017-01-17
DOI:
EISSN:
1439-7633
ISSN:
1439-4227
Source identifiers:
671895
Language:
English
Keywords:
Pubs id:
pubs:671895
UUID:
uuid:5a110e8d-b3fa-4c25-9edf-966d36a02ebe
Local pid:
pubs:671895
Deposit date:
2017-04-10

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