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z-STED Imaging and Spectroscopy to Investigate Nanoscale Membrane Structure and Dynamics

Abstract:

Super-resolution stimulated emission depletion (STED) microcopy provides optical resolution beyond the diffraction limit. The resolution can be increased laterally (xy) or axially (z). Two-dimensional STED has been extensively used to elucidate the nanoscale membrane structure and dynamics via imaging or combined with spectroscopy techniques such as fluorescence correlation spectroscopy (FCS) and spectral imaging. On the contrary, z-STED has not been used in this context. Here, we show that a...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.bpj.2020.04.006

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Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Oxford college:
University College
Role:
Author
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Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Role:
Author
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Publisher:
Biophysical Society Publisher's website
Journal:
Biophysical Journal Journal website
Volume:
118
Issue:
10
Pages:
2448-2457
Publication date:
2020-04-16
Acceptance date:
2020-04-06
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
Language:
English
Keywords:
Pubs id:
1102820
Local pid:
pubs:1102820
Deposit date:
2020-05-06

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