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GINS inactivation phenotypes reveal two pathways for chromatin association of replicative α and ε DNA polymerases in fission yeast

Abstract:

The tetrameric GINS complex, consisting of Sld5-Psf1-Psf2-Psf3, plays an essential role in the initiation and elongation steps of eukaryotic DNA replication, although its biochemical function is unclear. Here we investigate the function of GINS in fission yeast, using fusion of Psf1 and Psf2 subunits to steroid hormone-binding domain (HBD) to make GINS function conditional on the presence of β-estradiol. We show that inactivation of Psf1-HBD causes a tight but rapidly reversible DNA replicati...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1091/mbc.E08-04-0429

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Institution:
University of Oxford
Division:
MPLS
Department:
Zoology
Role:
Author
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Institution:
University of Edinburgh
Department:
Wellcome Trust Centre for Cell Biology
Role:
Author
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Institution:
University of Oxford
Division:
MPLS
Department:
Zoology
Role:
Author
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Institution:
St George's Hospital Medical School
Department:
Department of Biochemistry and Immunology
Role:
Author
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Institution:
"university of Edinburgh", "University of Copenhagen, Denmark"
Department:
Department of Biology
Role:
Author
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Funding agency for:
García, I
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Funding agency for:
Cotterill, S
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Funding agency for:
Kearsey, S
Publisher:
American Society for Cell Biology Publisher's website
Journal:
Molecular Biology of the Cell Journal website
Volume:
20
Issue:
4
Pages:
1213-1222
Publication date:
2009-02-01
DOI:
EISSN:
1939-4586
Language:
English
Keywords:
Subjects:
UUID:
uuid:5e4bc637-452c-4900-9097-5dff4e98d054
Local pid:
ora:2847
Deposit date:
2009-07-06

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