Journal article
GINS inactivation phenotypes reveal two pathways for chromatin association of replicative α and ε DNA polymerases in fission yeast
- Abstract:
-
The tetrameric GINS complex, consisting of Sld5-Psf1-Psf2-Psf3, plays an essential role in the initiation and elongation steps of eukaryotic DNA replication, although its biochemical function is unclear. Here we investigate the function of GINS in fission yeast, using fusion of Psf1 and Psf2 subunits to steroid hormone-binding domain (HBD) to make GINS function conditional on the presence of β-estradiol. We show that inactivation of Psf1-HBD causes a tight but rapidly reversible DNA replicati...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Authors
Funding
+ "Wellcome Trust", "Forskningsrådet for Natur og Univers and Novo Nordisk Fonden"
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Funding agency for:
MacNeill, S
Bibliographic Details
- Publisher:
- American Society for Cell Biology Publisher's website
- Journal:
- Molecular Biology of the Cell Journal website
- Volume:
- 20
- Issue:
- 4
- Pages:
- 1213-1222
- Publication date:
- 2009-02-01
- DOI:
- EISSN:
-
1939-4586
Item Description
- Language:
- English
- Keywords:
- Subjects:
- UUID:
-
uuid:5e4bc637-452c-4900-9097-5dff4e98d054
- Local pid:
- ora:2847
- Deposit date:
- 2009-07-06
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- Copyright holder:
- American Society for Cell Biology
- Copyright date:
- 2009
- Notes:
- Citation: Pai, C. C. et al. (2009). 'GINS inactivation phenotypes reveal two pathways for chromatin association of replicative α and ε DNA polymerases in fission yeast', Molecular Biology of the Cell, 20(4), 1213-1222. [Available at http://www.molbiolcell.org/].
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