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Journal article

Biotagging of specific cell populations in zebrafish reveals gene regulatory logic encoded in the nuclear transcriptome

Abstract:

Interrogation of gene regulatory circuits in complex organisms requires precise tools for the selection of individual cell types and robust methods for biochemical profiling of target proteins. We have developed a versatile, tissue-specific binary in vivo biotinylation system in zebrafish termed biotagging that uses genetically encoded components to biotinylate target proteins, enabling in-depth genome-wide analyses of their molecular interactions. Using tissue-specific drivers and cell-compa...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.celrep.2017.03.045

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
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Institution:
University of Oxford
Division:
MSD
Department:
RDM
Role:
Author
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Grant:
Basil O’Connor Award (#5-FY12-564
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Grant:
Fellowship (to V.C.-M.
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Grant:
Fellowship (PBSKP3_145791 to D.G.
Publisher:
Cell Press Publisher's website
Journal:
Cell Reports Journal website
Volume:
19
Issue:
2
Pages:
425-440
Publication date:
2017-04-11
Acceptance date:
2017-03-13
DOI:
ISSN:
2211-1247
Source identifiers:
690820
Language:
English
Keywords:
Pubs id:
pubs:690820
UUID:
uuid:5f9b33f9-722a-4bc3-adef-2e1c03382e51
Local pid:
pubs:690820
Deposit date:
2017-06-13

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