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Thesis

Investigating high-affinity non-covalent protein-ligand interaction via variants of streptavidin

Abstract:

The Streptomyces avidinii protein streptavidin binds the small molecule biotin (vitamin H / B₇) with extraordinary stability, resulting in the streptavidin-biotin interaction being one of the strongest non-covalent interactions known in nature (Kd ~ 10-14 M). The stable and rapid biotin-binding, together with high resistance to heat, pH and proteolysis, has given streptavidin huge utility, both in vivo and in vitro. Accordingly, streptavidin has beco...

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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Research group:
Dr Mark Howarth
Oxford college:
Somerville College
Role:
Author

Contributors

Division:
MSD
Department:
Biochemistry
Role:
Supervisor
Publication date:
2011
Type of award:
DPhil
Level of award:
Doctoral
Awarding institution:
University of Oxford
Language:
English
Keywords:
Subjects:
UUID:
uuid:631c65ed-08d9-484e-a8df-309a4c95df45
Local pid:
ora:5987
Deposit date:
2012-01-06

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