Ni(2+) affects dopamine uptake which limits suitability as inhibitor of T-type voltage-gated Ca(2+) channels.

Journal article

Neuronal T-type voltage-gated Ca(2+) channels are reported to have physiological roles that include regulation of burst firing, Ca(2+) oscillations, and neurotransmitter release. These roles are often exposed experimentally by blocking T-type channels with micromolar Ni(2+). We used Ni(2+) to explore the role of axonal T-type channels in dopamine (DA) release in mouse striatum, but identified significant off-target effects on DA uptake. Ni(2+) (100 μM) reversibly increased electrically evoked...

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Authors: Brimblecombe, K; Cragg, S

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: American Chemical Society Publisher's website
• Journal: ACS chemical neuroscience Journal website
• Volume: 6
• Issue: 1
• Pages: 124-129
• Publication date: 2015-01-01
• DOI: 10.1021/cn500274g
• EISSN: 1948-7193
• ISSN: 1948-7193
• Language: English
• Keywords: Dose-Response Relationship, Drug; Animals; Acetylcholine; Dopamine; Calcium Channels, T-Type; Biophysics; In Vitro Techniques; Corpus Striatum; Action Potentials; Dopamine Agents; Calcium Channel Blockers; Male; Electrochemical Techniques; Electric Stimulation; Dihydro-beta-Erythroidine; Nickel; Mice; Analysis of Variance; Mice, Inbred C57BL; Neurons