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Structure of V-ATPase from the mammalian brain

Abstract:

In neurons, the loading of neurotransmitters into synaptic vesicles uses energy from proton-pumping vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases). These membrane protein complexes possess numerous subunit isoforms, which complicates their analysis. We isolated homogeneous rat brain V-ATPase through its interaction with SidK, a Legionella pneumophila effector protein. Cryo-electron microscopy allowed the construction of an atomic model, defining the enzyme's ATP:proton rati...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1126/science.aaz2924

Authors


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Role:
Author
ORCID:
0000-0001-5625-1034
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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Oxford college:
Exeter College
Role:
Author
ORCID:
0000-0001-7829-5505
More by this author
Role:
Author
ORCID:
0000-0003-0566-2209
Wellcome Trust More from this funder
Publisher:
American Association for the Advancement of Science Publisher's website
Journal:
Science Journal website
Volume:
367
Issue:
6483
Pages:
1240-1246
Publication date:
2020-03-13
Acceptance date:
2020-02-12
DOI:
EISSN:
1095-9203
ISSN:
0036-8075
Pmid:
32165585

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