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High spatial and temporal resolution Ca2+ imaging of myocardial strips from human, pig and rat

Abstract:

Ca2+ handling within cardiac myocytes underpins coordinated contractile function within the beating heart. This protocol enables high spatial and temporal Ca2+ imaging of ex vivo multicellular myocardial strips. The endocardial surface is retained, and strips of 150–300-µm thickness are dissected, loaded with Ca2+ indicators and mounted within 1.5 h. A list of the equipment and reagents used and the key methodological aspects allowing the use of this technique on strips from any chamber of th...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41596-021-00590-6

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Grant:
PG/14/58/30998
FS/13/16/30199
Publisher:
Springer Nature Publisher's website
Journal:
Nature Protocols Journal website
Volume:
16
Pages:
4650–4675
Publication date:
2021-08-16
Acceptance date:
2021-06-14
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
Language:
English
Keywords:
Pubs id:
1190771
Local pid:
pubs:1190771
Deposit date:
2021-08-13

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