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Sensorless adaptive optics for isoSTED nanoscopy

Abstract:

The presence of aberrations is a major concern when using fluorescence microscopy to image deep inside tissue. Aberrations due to refractive index mismatch and heterogeneity of the specimen under investigation cause severe reduction in the amount of fluorescence emission that is collected by the microscope. Furthermore, aberrations adversely affect the resolution, leading to loss of fine detail in the acquired images. These phenomena are particularly troublesome for super-resolution microscop...

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Publication status:
Published
Peer review status:
Peer reviewed

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Files:
Publisher copy:
10.1117/12.2288973

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Centre for Neural Circuits and Behaviour
Role:
Author
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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Oxford Ludwig Institute
Role:
Author
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More from this funder
Grant:
095927/A/11/Z, 095927/B/11/Z, 203285/B/16/Z, 203285/C/16/Z
G. Harold and Leila Y. Mathers Foundation More from this funder
Publisher:
Society of Photo-Optical Instrumentation Engineers Publisher's website
Journal:
Progress in Biomedical Optics and Imaging - Proceedings of SPIE Journal website
Volume:
10502
Article number:
1050206
Host title:
Progress in Biomedical Optics and Imaging - Proceedings of SPIE: Adaptive Optics and Wavefront Control for Biological Systems IV
Publication date:
2018-02-23
Acceptance date:
2017-09-25
DOI:
ISSN:
1605-7422
Source identifiers:
843451
ISBN:
9781510614895
Keywords:
Pubs id:
pubs:843451
UUID:
uuid:6f0befaa-fb0e-4f46-8bc7-0d454b236b3d
Local pid:
pubs:843451
Deposit date:
2018-05-22

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