Functional human artificial chromosomes are generated and stably maintained in human embryonic stem cells.

Journal article

We present a novel and efficient non-integrating gene expression system in human embryonic stem cells (hESc) utilizing human artificial chromosomes (HAC), which behave as autonomous endogenous host chromosomes and segregate correctly during cell division. HAC are important vectors for investigating the organization and structure of the kinetochore, and gene complementation. HAC have so far been obtained in immortalized or tumour-derived cell lines, but never in stem cells, thus limiting their...

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Authors: Mandegar, M; Moralli, D; Khoja, S; Cowley, S; Chan, D

• Publication status: Published
• Journal: Human molecular genetics
• Volume: 20
• Issue: 15
• Pages: 2905-2913
• Publication date: 2011-08-01
• DOI: 10.1093/hmg/ddr144
• EISSN: 1460-2083
• ISSN: 0964-6906
• Language: English
• Keywords: Cell Line; Humans; Cell Differentiation; Chromosomes, Artificial, Human; Neurons; Herpesvirus 1, Human; Flow Cytometry; Embryonic Stem Cells; Fluorescent Antibody Technique