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Dataset

3D histology reconstruction with external blockface reference. Full mouse heart, 600 sections

Alternative title:
3D reconstruction of Picro sirius red histology data using Transformation Diffusion Reconstruction
Version:
1.0

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Authors/Creators


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Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Sub department:
Institute of Biomedical Engineering
Role:
Depositor, Researcher, Creator
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Department:
Heart Science Centre, National Lung and Heart Institute, Imperial College London, Harefield UB9 6JH, UK.
Role:
Creator
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Department:
Heart Science Centre, National Lung and Heart Institute, Imperial College London, Harefield UB9 6JH, UK
Role:
Creator
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Department:
Heart Science Centre, National Lung and Heart Institute, Imperial College London, Harefield UB9 6JH, UK
Role:
Creator
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Department:
Department of Physiology and Biophysics, Dalhousie University, Halifax, NS B3H 4R2, Canada
Role:
Creator
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Contributors

Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Human Genetics Wt Centre
Role:
Principal Investigator (PI)
Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Sub department:
Institute of Biomedical Engineering
Role:
Principal Investigator (PI)
More from this funder
Funding agency for:
Schneider, J
Grant:
FS/11/50/29038
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Funding agency for:
Kohl, P
Grant:
FS/12/17/29532
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Publisher:
Institute for Experimental Cardiovascular Medicine, University of Freiburg; Institute of Biomedical Engineering, Department of Engineering Science and Wellcome Trust Centre for Human Genetics, University of Oxford; Heart Science Centre, National Lung and Heart Institute, Imperial College London
Publication date:
2017
Version number:
1.0
DOI:
Keywords:
Subjects:
Documentation:
Mouse heart Picro sirius red histology was reconstructed using the Transformation Diffusion Reconstruction (TDR) method in (Casero et al. 2017). Histology slices were downsampled by a factor of x24.6 down to blockface pixel size, and pre-aligned to the blockface using rigid registration, followed by rigid TDR refinement (M=150 stack sweeps) and B-spline ATDR refinement (M=80). We then applied the low resolution refinement to the original histology downsampled only by a factor of 2. In this ... Expand documentation
UUID:
uuid:716fe2ef-f965-40ff-8da4-8a5ad48f4aea
Deposit date:
2017-03-28

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