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Journal article

Microscope calibration using laser written fluorescence

Abstract:
There is currently no widely adopted standard for the optical characterisation of fluorescence microscopes. We used laser written fluorescence to generate two- and threedimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written patterns to measure the lateral resolution, illumination uniformity, lens distortion and colour plane alignment using confocal and structured illumination fluorescence microscopes.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1364/OE.26.021887

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Institution:
University of Oxford
Division:
Maths, Physical & Life Sciences
Department:
Biochemistry
Role:
Author
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Institution:
University of Oxford
Division:
Maths, Physical & Life Sciences
Department:
Engineering Science
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Maths, Physical & Life Sciences
Department:
Biochemistry
Role:
Author
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Publisher:
Optical Society of America Publisher's website
Journal:
Optics Express Journal website
Volume:
26
Issue:
17
Pages:
21887-21899
Publication date:
2018-08-08
Acceptance date:
2018-08-01
DOI:
EISSN:
1094-4087
Source identifiers:
892188
Pubs id:
pubs:892188
UUID:
uuid:76bef4be-f1d2-4f4a-8d76-7c3d1a889fe6
Local pid:
pubs:892188
Deposit date:
2018-08-02

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