Development of spontaneous isopeptide bond formation for ligation of peptide tags

Thesis

Peptide tags are ubiquitous in the life sciences, with roles including purification and selective labeling of proteins. Because peptide tags are small they have a limited surface area for binding and hence usually form low affinity protein interactions. These weak interactions limit the uses of peptide tags in cases that require resistance to forces generated with macromolecular architectures or protein motors. Hence a way to create a covalent interaction with a peptide tag would be useful...

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Authors: Fierer, J; Jacob Orry Fierer

• Publication date: 2014
• Type of award: DPhil
• Level of award: Doctoral
• Awarding institution: Oxford University, UK
• Language: English
• Keywords: SpyCatcher; SpyTag; Split-Protein; SpyLigase; Protein Ligation
• Subjects: Medical sciences; Chemical biology; Protein chemistry; Blood; Life Sciences; Biosensors; Protein folding; Oncology; Materials Sciences; Molecular biophysics (biochemistry); Nanomaterials; Biochemistry; Nano-biotechnology