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Analysis of nitroxide spin label motion in a protein-protein complex using multiple frequency EPR spectroscopy.

Abstract:

X- and W-band EPR spectra, at room and low temperatures, are reported for nitroxide spin labels attached to cysteine residues selectively introduced into two proteins, the DNase domain of colicin-E9 and its immunity protein, Im9. The dynamics of each site of attachment on the individual proteins and in the tight DNase-Im9 complex have been analysed by computer simulations of the spectra using a model of Brownian dynamics trajectories for the spin label and protein. Ordering potentials have be...

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Publisher copy:
10.1016/j.jmr.2006.12.009

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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
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Journal:
Journal of magnetic resonance (San Diego, Calif. : 1997)
Volume:
185
Issue:
2
Pages:
191-203
Publication date:
2007-04-01
DOI:
EISSN:
1096-0856
ISSN:
1090-7807
Source identifiers:
310224
Language:
English
Keywords:
Pubs id:
pubs:310224
UUID:
uuid:8bef6104-a15e-4074-8acc-2cff6b8c4be5
Local pid:
pubs:310224
Deposit date:
2013-11-16

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