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Preserving the photoswitching ability of standard fluorescent proteins for correlative in-resin super-resolution and electron microscopy

Abstract:

There are many different correlative light and electron microscopy (CLEM) techniques available. The use of super-resolution microscopy in CLEM is an emerging application and while offering the obvious advantages of improved resolution in the fluorescence image, and therefore more precise correlation to electron microscopy (EM) ultrastructure, it also presents new challenges. Choice of fluorophore, method of fixation, and timing of the fluorescence imaging are critical to the success of super-...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/bs.mcb.2017.04.001

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
Publisher:
Elsevier Publisher's website
Volume:
140
Pages:
49-67
Host title:
Methods in Cell Biology
Publication date:
2017-05-05
Acceptance date:
2017-05-05
DOI:
ISSN:
0091-679X
Pmid:
28528641
Source identifiers:
696554
Language:
English
Keywords:
Pubs id:
pubs:696554
UUID:
uuid:a26058dd-f52b-4a18-bf1b-618404b97e80
Local pid:
pubs:696554
Deposit date:
2019-08-21

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