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Sensorless adaptive optics with a laser free spinning disk confocal microscope

Abstract:

Specimen induced aberrations can have detrimental effects in all types of high-resolution microscope. In this study, we present a sensorless technique that uses a deformable mirror (DM) to correct aberrations of both the system and sample. Using a laser-free confocal microscope, with patterned disk illumination and detection. The system is based on a commercial confocal module (Clarity, Aurox Ltd., UK) that uses Light Emitting Diode (LED) illumination to obtain optically sectioned 3D images. ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1117/12.2542959

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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Role:
Author
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Publisher:
Society of Photo-optical Instrumentation Engineers Publisher's website
Journal:
Proceedings of SPIE Journal website
Volume:
11248
Publication date:
2020-02-17
Acceptance date:
2019-12-02
Event title:
Adaptive Optics and Wavefront Control for Biological Systems VI
Event location:
San Francisco, California, USA
Event start date:
2020-02-01T00:00:00Z
Event end date:
2020-02-06T00:00:00Z
DOI:
EISSN:
1996-756X
ISSN:
0277-786X
ISBN:
9781510632592
Language:
English
Keywords:
Pubs id:
1099622
Local pid:
pubs:1099622
Deposit date:
2020-06-03

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