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The β-lactamase assay: harnessing a FRET biosensor to analyse viral fusion mechanisms

Abstract:

The β-lactamase (BlaM) assay was first revealed in 1998 and was demonstrated to be a robust Förster resonance energy transfer (FRET)-based reporter system that was compatible with a range of commonly used cell lines. Today, the BlaM assay is available commercially as a kit and can be utilised readily and inexpensively for an array of experimental procedures that require a fluorescence-based readout. One frequent application of the BlaM assay is the measurement of viral fusion – the moment at ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Files:
Publisher copy:
10.3390/s16070950

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Human Genetics Wt Centre
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Human Genetics Wt Centre
Role:
Author
Publisher:
MDPI Publisher's website
Journal:
Sensors Journal website
Publication date:
2016-06-23
Acceptance date:
2016-06-20
DOI:
EISSN:
1424-8220
ISSN:
1424-8220
Source identifiers:
629060
Keywords:
Pubs id:
pubs:629060
UUID:
uuid:cbeefacc-96f7-4c7a-8c2e-5555a2222c61
Local pid:
pubs:629060
Deposit date:
2016-06-20

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